Development and characterization of 37 SNP markers for the largemouth bass (Micropterus salmoides) by using PCR–RFLP method

نویسندگان

چکیده

Largemouth bass (Micropterus salmoides) is an economically important species in China. Contrary to its rapidly increasing yield during the last decades, domestic genetic diversity of largemouth has gradually declined. In this study, 37 single nucleotide polymorphism (SNP) markers were developed based on genotyping-by-sequencing data and characterized by genotyping 32 individuals using PCR–restriction fragment length method. The effective number alleles (Ne), observed heterozygosity (Ho), expected (He), polymorphic information content (PIC) these SNPs ranged from 1.168 1.998, 0.156 0.844, 0.146 0.507, 0.134 0.375, respectively. Totally, five loci deviated significantly Hardy–Weinberg equilibrium (p < 0.05), while there existed no linkage disequilibrium at all loci. These novel will lay foundation for future rationally excavation utilization germplasm resources M. salmoides.

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ژورنال

عنوان ژورنال: Conservation Genetics Resources

سال: 2021

ISSN: ['1877-7260', '1877-7252']

DOI: https://doi.org/10.1007/s12686-021-01229-0